SKU: 70826849829

E2 (Estradiol) ELISA Kit

Sale price$202.50 Regular price$225.00
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Ships within 48 hours · Estimated delivery Jul 14 - Jul 19

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Description

E2 (Estradiol) ELISA KitThe E2 (Estradiol) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate reaction is

The E2 (Estradiol) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 70826849829

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