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Description
Caspase 6 Activity Assay KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 6 enzyme activity: 1. Take out an appropriate amount of Ac-VEID-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-VEID-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-VEID-pNA (2mM). Add Ac-VEID-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 6 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 6, it can catalyze the substrate Val Glu Ile Asp p nitroanilide (Ac VEID pNA) to produce yellow pNA (p-nitroanilide), which can be used to detect the activity of Caspase 6 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 6 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader, spectrophotometer |
| Synonym | Mch-2,caspase-6,caspase 6 |
| Description | caspase 6 Activity Detection Kit is a kit that detects caspase 6 enzyme activity or purified caspase 6 enzyme activity in cell or tissue lysates by spectrophotometry. |
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4.5 ★★★★★
Based on 431 reviews
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Product Reviews
★★★★★ 1
My Monster Dogs destroyed these balls
Color: Glow in the dark II, Size: Large
I was so excited to get two of these balls for my doodle puppies to have to play with . THEY DESTROYED THEM IN ALL OF TEN MINUTES. I don't believe that there was anything wrong with the balls. They were beautiful, high quality balls. Just not durable enough for my doodles.
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Reviewed in the United States on March 25, 2026
★★★★★ 3
Doesn't glow in the dark but my dog still enjoyed
Color: Glow in the dark Ⅳ, Size: Medium
Great
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Reviewed in the United States on May 4, 2026
★★★★★ 5
Sturdy, well made, puppy & senior dog approved
Color: Glow in the dark I, Size: Medium
Pet approved !!!! Our puppy immediately grabbed it by the straps and took off!! It is well made and has handled some serious chewing in the last 24 hours. We love the glow in the dark because puppy playtime does not stop when it gets dark out!! It came with a pump and was very easy to assemble and inflate. This toy would be great for a pet owner that struggles with grasp & grip strength, the straps are absolutely genius.
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Reviewed in the United States on February 10, 2026
★★★★★ 5
Fun, Durable Ball, But Straps Could Be Tougher
Color: Glow in the dark I, Size: Large
I bought the QDAN Glow in the Dark Dog Toys Soccer Ball for my German Shepherd, who absolutely loves it. The glow-in-the-dark feature is great for evening play, adding an extra layer of fun to our outdoor interactions. The ball itself has proven to be remarkably durable, standing up well to the rough play that comes with a large, active dog.
However, the straps did not fare as well. My GSD managed to bite through most of them relatively quickly. While this hasn’t stopped us from enjoying the ball, it does mean that some of the interactive potential of the toy is lost. The ball continues to be a hit on its own, thanks to its resilience and the glow feature that keeps my dog engaged.
If the straps were as durable as the ball itself, this would be a perfect outdoor toy for energetic dogs. As it stands, it’s still a good purchase for anyone looking for a sturdy, engaging toy for their pet, especially if nighttime play is a routine.
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Reviewed in the United States on September 25, 2024
★★★★★ 5
Pup loves this!
Size: 6 Inch, Color: Red
This soccer ball is so cute. My dog loves to play with these. The straps are durable, easy to pump up (they come with a pump), good value for the money since they tend to last awhile, at least for us. The straps are durable but could be an issue if your dog is an aggressive chewer. They stay pumped up for quite awhile and just need to be topped off once in awhile to keep the bounce there. We keep them outside even in the winter and they do just fine. HIghly recommend as a good play toy.
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Reviewed in the United States on December 16, 2025
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