Human IL-12/IL-23 p40 ELISA Kit
SKU: 50423812708

Human IL-12/IL-23 p40 ELISA Kit

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Description

Human IL-12/IL-23 p40 ELISA KitProduct Specification Species Reactivity Human Synonym Human IL 12 ELISA kit, IL 23 p19 IL 12 p40, IL23, IL 23, IL 23A, IL 23 A, IL 23p19, IL 23p19 IL 12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin 23 subunit alpha, Interleukin 23 subunit p19, JKA3 induced upon T cell activation, MGC79388, SGRF, SGRFIL 23 subunit alpha, Description Detection principle: Double antibody sandwich ELISA was used in this

Product Specification

Species Reactivity Human
Synonym Human IL-12 ELISA kit, IL-23 p19/IL-12 p40, IL23, IL-23, IL-23A, IL-23-A, IL-23p19, IL-23p19/IL-12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T-cell activation, MGC79388, SGRF, SGRFIL-23 subunit alpha,
Description

Detection principle:

Double antibody sandwich ELISA was used in this experiment. Anti-human IL-12/IL-23p40 monoclonal antibody is coated on a microplate, and IL-12/IL-23p40 in samples and standards will bind to the antibody immobilized on the plate, and the free components will be washed away; Horseradish peroxidase-labeled anti-human IL-12/IL-23 p40 polyantibody was added and unbound antibody was washed away; Adding a substrate solution (chromogenic agent), the color of the solution is proportional to the bound target protein; A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).

Test Type:Double antibody sandwich method

Form:Pre-coated 96-well plate

Test Sample Type:Cell supernatant, serum, plasma

Sample Load:100ul

Kit Components:

A copy of the pre-coated 96-well plate, standard, anti-human IL-12/IL-23 p40 detection antibody, buffer for dilution, chromogenic solution (A, B), wash solution, stop solution, SA-HRP, plate sealing membrane, and instructions.

Sensitivity:3.5 pg/mL

Detection range:62.5-4,000 pg/mL

Recovery Range:89-114%

Save Method:2-8℃

Standard curve plot

Background:

IL-12, also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine mainly produced by antigen-presenting cells (monocytes/macrophages, dendritic cells, and B lymphocytes). IL-12 has a variety of effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production, and Th1 subset development.

IL-12 is a 70 kDa (p70) heterodimeric glycoprotein linked by disulfide bonds, consisting of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subbases do not possess IL-12 activity in themselves, but homodimers of p40 have been shown to bind IL-12 receptors and are IL-12 antagonists.

The genes of human p40 and p35 located on chromosomes 5 and 3, respectively, are independently regulated. The expression of p35 mRNA has been found to be almost ubiquitous, however, the p35 subunit was not detected in the culture supernatant of cells expressing p35 or p35 and p40 mRNA only. The p40 mRNA expression level was higher in cells expressing both p35 and p40 mRNA, and together with the heterodimeric IL-12 p70, a free p40 subunit unrelated to the p35 subunit was secreted. Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist in monomeric form. The content of free p40 far exceeds p70 in the supernatants of various activated human monocytes, and the levels of p70 measured by bioassays are consistent with those measured using p70-specific immunoassays, suggesting that p40 monomers are not potent IL-12 antagonists. Currently, the physiological role of the free p40 subunit is unclear.

 

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SKU: 50423812708

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Tasha U
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★★★★★ 4
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We use these on my husband’s cracked feet in the winter and put on a thick cream under these socks. They are nice because hair, dirt and that kind of stuff doesn’t stick to the outside. I do wish them came in more sizes and colors. They were a little small for my husband, but still worked good enough. My husband isn’t thrilled about wearing pink socks around the house. At least it’s just around the house and not out in public though.
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Be aware! Once they are on, do not walk! 😂
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I absolutely love these things. But the first time I put them on in the living room. The walk to my bedroom was VERY interesting 😂😂😂 I now know I need to put them on and hang out for a bit, or simply go to bed. They do exactly what I need them to do. Feet feel great afterwards! With the right lotion, only need to use one every couple of weeks.
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Alexandria, US
★★★★★ 5
Transformed my feet
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After trying several other brands that actually my size did not have the luxury feeling of these. T although x large they have the BEST feel and function. I put lotion on then slip them on and within a few minutes my dry cracked heals are soft. Really a nice product.
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Tina Taylor
Cuba, US
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Sparkly good value for money
Color: Silver
Very pretty and sparkly. Use lotion before hand and it sticks even better.
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