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Description
Human TNF-α ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human TNF antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human TNF-α antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa. TNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.6 ★★★★★
Based on 704 reviews
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Product Reviews
★★★★★ 5
HIGHLY RECOMMENDED. Spells great
I use this product everyday and have used an entire set. They smell great and get you clean, there is a loofa bag included where you put the soap inside and makes scrubbing convenient.
Recommend if you want to feel clean and smell really good.. this is the set you can use. I know the ladies like it a lot. I get compliments all the time.
One lady told me "You smell like money"... LOL.. true story.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 2, 2025
★★★★★ 4
Great scent, but leaves a film on my skin.
I was expecting bars of soap with more of a peppermint/eucalyptus fragrance and I just could not relate those fragrances to what the soap actually smells like. The Viking Revolution soap doesn't smell bad per se, and actually for me is rather fragrance neutral like you might find with the more sterile hospital and clinic type soaps.
The lather factor for the Viking Revolution soap is mild to moderate in our hard water. It doesn't overdo lathering up but does lather up enough to let you know it is soap and is rather pleasing when rinsing it off where with some soaps you rinse and rinse and still need to rinse more to get the lather off your skin.
I wash my hands and face many times throughout the day and night and so far the Viking Revolution soap has done a great job in leaving me feel a bit more refreshed and clean. I have noticed a bit of moisturizing of my face and hands being much softer after use, so the Viking Revolution soap does seem to come through with that feature. I handle and am around livestock and animals quite often and did notice that the soap seemed to cut through the grime pretty easily. I haven't tried the Viking Revolution as a body wash type soap. The square bars are just about a perfect fit for my hand and are easy to hold onto. I will use one bar for the shower and keep the bar in a drip rack where it has a chance to dry out after use.
I do really like the presentation of the soap itself and it's a nice touch that each bar comes individually wrapped and includes a sticker and a small card. A rather attractive package if you are giving it for a gift. I'm more favorable of recommending this soap than not recommending it. The only reason I am giving 4 stars is for the fragrances not really being detectable as peppermint and eucalyptus.I do think I would buy this soap again though, fragrance or not.
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Reviewed in the United States on January 14, 2024
★★★★★ 5
Awesome soap!
I love this soap. It was a little small, ut was great quality. If smelled good and did not break me out. I have very sensitive skin and this was very gentle on my skin. I usually use caswell and Massey soap and this soap is every bit as good as my normal c&m soap. Very good soap. I Will definitely will buy again.
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Reviewed in the United States on June 2, 2024
★★★★★ 3
Scent is too perfumey
This soap pack is ok for the price, but the bars don't lather very well. They do have a good level of moisturizer that doesn't feel too oily, but my big complaint is the scents. The black one is ok, but the others are like cheap perfume. And I mean CHEAP perfume. It actually hurts my nose. I have used higher end soaps with a beautiful scent that is subtle and natural. This is not that. Overall not a bad product for the price, but don't expect to smell like a $1 perfume. Yuck
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Reviewed in the United States on May 28, 2024
★★★★★ 5
Great scents and clean feel.”
This 4-pack (Sandalwood Amber, Mint Olive, Turmeric Citrus, Activated Charcoal) hits the mark. Natural, handmade, and leaves skin feeling fresh without drying out.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 20, 2025
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