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Description
Mouse FGL2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect specimens using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for analysis or store at 20C
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect specimens using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Tissue homogenization: Rinse tissue with pre-chilled PBS (0.01M, pH=7.4) to remove residual blood (lysed red blood cells in the homogenate may affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse the tissue cells, the homogenate can be sonicated or repeatedly freeze-thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells are gently washed with ice-cold PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. The harvested cells are washed three times with ice-cold PBS and resuspended in 150-200µL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, the PBS volume can be reduced appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C and remove the supernatant for testing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes and remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Fibrinogen Like Protein 2 (FGL2). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Fibrinogen Like Protein 2 (FGL2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Fibrinogen Like Protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Fibrolin (FGL2) is a protein encoded by the FGL2 gene. It is a 439-amino acid secreted protein that is similar to the β- and γ-chains of fibrinogen. Its carboxyl terminus is composed of a fibrinogen-related domain (FRED). It forms a quaternary complex stabilized by interchain disulfide bonds. It may play a role in physiological functions at mucosal sites. It exhibits multiple roles in vivo and is an important immunomodulator of both innate and adaptive responses. This protein is present on the surface of macrophages and endothelial cells as a type II transmembrane protein (with the carboxyl terminus on the outer side of the plasma membrane) and is constitutively secreted by CD4+ and CD8+ T cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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Product Reviews
★★★★★ 5
Comfortable
Color: Black,navy,burgundy,brown, Size: Medium
I have had a lifetime of different briefs, some name brand, some generic.
I am 165 lbs, wear Medium, and these are closest to feeling commando of anything I’ve ever worn.
Comfortable, not too tight waist and legs, and yes silky touch yet not slinky.
Maybe not for support wear, but casual and cool comfort, the “Cat’s meow”.
I just came on to order some more
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Reviewed in the United States on April 30, 2026
★★★★★ 3
Great fabric - pouch too small!
Color: Black, Size: X-Large
First, the fabric used is wonderfully soft and comfortable. It;s the first thing noticed when package was opened. Strange, but the elastic waistband is about 1/2", very similar to a woman's style panty. But it's comfortable and easily acceptable.
The main problem is that the pouch that holds your "stuff" in place, is oddly and unnaturally small. Small enough where this average man's "stuff" tends to fall out of the pouch on one side or the other. I sleep in my underwear, and every time I did with these, I awoke with the front pouch off to the side and everything down there was hanging outside of the underwear.
They also seem to do well through a wash cycle. However, if the main reason for wearing underwear is to keep your stuff in place, these fail from the beginning.
I ordered another brand from overseas, and they had the exact same problems as these! I wonder if that's just how they make them outside of the USA.
The Bottom Line?
Uncle Steve Disapproved!
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Reviewed in the United States on October 30, 2025
★★★★★ 5
Premium cotton underwear at a modest price. Highly recommend!
Color: Multicolora6-4 Pack, Size: X-Large
Bought my husband 2 variety packs of these, and they are still great over 2 years in. High quality cotton briefs are breathable, colors are vibrant, and the fit is spot on. A good value at this price point compared to designer brands, and the product overall exceeds expectations in terms of comfort level, durability and appearance.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Comfort, Support, and Quality – The Perfect Boxer Briefs
Color: White, Size: X-Large
These men’s boxer briefs are everything I look for in everyday underwear. The fit is excellent — snug where it needs to be without feeling restrictive, and the fabric has just the right amount of stretch for all-day comfort.
The material is soft, breathable, and holds up great after multiple washes. No fading, no sagging, and most importantly — no riding up. The waistband is strong yet comfortable, staying in place without digging into the skin.
What really stands out is how well they balance support and freedom of movement. Whether I’m working out, at the office, or just relaxing at home, these boxer briefs stay comfortable and keep everything in place.
Great value for the price and a dependable choice for everyday wear. I’ll definitely be buying more. Highly recommend!
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Reviewed in the United States on August 22, 2025
★★★★★ 5
Nice underwear to lounge around the House
Color: Multicolora8-4 Pack, Size: X-Large
Nice fit. They hug your thigh and the material is silky soft.
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Reviewed in the United States on May 31, 2026