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Description
Mouse β-Hex ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. 5. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). 6. Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. 7. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Hexosaminidase B Beta (β-Hex) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Hexosaminidase B Beta (β-Hex) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Hexosaminidase B Beta ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Hexosaminidase (β-acetylaminodeoxyhexosidase, N-acetyl-β-D-hexosidase, N-acetyl-β-hexosidase, N-acetylhexosidase, β-hexosidase, β-acetylhexosidase, β-DN-acetylhexosidase, β-N-acetyl-D-hexosidase. β-N-acetylglucosaminidase, hexosaminidase A, N-acetylhexosaminidase, β-D-hexosaminidase) is an enzyme involved in the hydrolysis of the terminal N-acetyl-D-hexosamine residue in N-acetyl-β-D-hexosamine. Elevated hexosaminidase levels in blood or urine are considered a biomarker for relapse during treatment for alcoholism. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 1772 reviews
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Product Reviews
★★★★★ 2
Cheap broken box and no bag, allow pieces to fall out and are now missing….
Color: Fifa Mascot 2, Size: 26PCS, Color: Fifa Mascot 2, Size: 26PCS
Tiny little puzzle with no picture and missing pieces….
What you should receive is a very small box, a little larger than a lighter or the size of a key fob. The box is very light weight and flimsy. Mine arrived crushed on the bottom corner and ripped. This is similar to a candy box. Same type and weight. The pieces are not in a bag at all so when my box arrived in the condition it was in, and nothing sealed, of course pieces are missing. There are supposed to be to be 26 and I don’t have but half. They all fell out of the box like candy falls out of a broken box.
The puzzle itself is done by laser and the pieces I do have fit together well but, the color is off. I believe they use a stain and the ink has spread into the other pieces next to them. The color is deep and intense and most is accurately done but some pieces have multiple colors that are not in the right place. Some pieces I can’t tell which side is the front and which side is the back. The pieces are also so small, with so much information crammed on, I can’t make it out as I work on it or once it’s completed the best I can with only part of the pieces. I am disappointed with the whole thing and can only say the laser cutting was done well. Other than that, I wouldn’t order again, especially for a souvenir.
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Reviewed in the United States on April 18, 2026
★★★★★ 3
Durable Wooden Puzzle, but the Visuals are Disappointing
Color: Fifa Mascot 1, Size: 26PCS, Color: Fifa Mascot 1, Size: 26PCS
I picked this up to get ready for the 2026 World Cup hype. While it’s a cool idea for a collectible, the final result is a bit of a letdown visually.
The Material
The best thing about this puzzle is that it’s made of wooden pieces rather than the typical thin cardboard you find in cheap puzzles. This gives it a nice weight and makes it feel much more durable. The pieces fit together well and it feels like it will last a long time.
The Problem: Distracting Black Outlines
The main reason for the 3-star rating is the printing and cut quality. As you can see in my photos, the black outlines around every single piece are way too noticeable.
Instead of the pieces blending together to create a smooth, clean image of the World Cup graphics, the whole thing looks "choppy" and fragmented.
Up close, the black seams really take away from the design, making the overall picture look less premium than it does in the stock photos.
Final Verdict
If you just want a quick, sturdy wooden activity for a soccer fan, this is fine. But if you’re looking for a high-quality display piece to show off your 2026 excitement, the heavy black lines might bother you. It’s a decent "mid-tier" collectible, but it could have been much better with a more seamless print.
Pros:
Solid wooden construction (much better than cardboard).
Officially branded 2026 World Cup item.
Easy to handle pieces.
Cons:
Heavy black outlines break up the image.
Doesn't look very "clean" once fully assembled.
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Love the flavor in my coffee.
Flavor Name: Vanilla
This taste so good and adds delightful flavor to my coffee. I love the pump on the bottle. I love the size of the bottle. It is a great price and quality all the way around. It mixes very well with my coffee. It lasts well, since you don't need much. I love the syrup, it is not watery.
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Reviewed in the United States on February 13, 2026
★★★★★ 5
Vanilla Syrup
Flavor Name: Vanilla
So tasty. Put in my ice coffee and it is perfect. Large bottle but just the right size for me using it daily. Highly recommend.
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Reviewed in the United States on April 17, 2026
★★★★★ 5
Wonderful for London Fogs
Flavor Name: Vanilla
This was a really good-tasting product! I personally use it to make London fogs, and it is perfect for this goal of mine. I do think the pump doesn't spray the syrup as "laminar" as I would like, which causes it to splash onto the table around my mug, but it's still a solid product despite this issue.
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Reviewed in the United States on April 26, 2026