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Description
Caspase 4 Activity Detection KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 4 enzyme activity: 1. Take out an appropriate amount of Ac-LEVD-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-LEVD-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-LEVD-pNA (2mM). Add Ac-LEVD-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 4 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 4, it can catalyze the substrate Leu Glu Val Asp p nitroanilide (Ac LEVD pNA) to produce yellow pNA (p-nitroaniline), which can be used to detect the activity of Caspase 4 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 4 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader or spectrophotometer |
| Synonym | caspase-4,caspase 4 |
| Description | caspase 4 Activity Detection Kit is a kit that detects caspase 4 enzyme activity or purified caspase 4 enzyme activity in cell or tissue lysates by spectrophotometry. |
| Storage Temp. | When stored at-20 °C, Ac-LEVD-pNA and pNA need to be stored in the dark. |
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4.6 ★★★★★
Based on 1315 reviews
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Product Reviews
★★★★★ 5
Labrador Retriever Fun Toy
Color: Yellow
Excellent for older Labrador Retriever with a soft mouth. 2 levels of movements. Colorful. Recharge quickly too, cable included. 2 velcro type covers.
Fairly chew resistant, watch your dog, as with any toy! Good size, no worries about my Labrador swallowing it!
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Reviewed in the United States on April 23, 2026
★★★★★ 5
Great engaging toy for my border collie
Color: Blue
I have an 8 yr old border collie with an injured rear hock, so she can't chase the Frisbee any longer. This toy, when set on high, kicks her hunting instincts in, and she chases it, brings it back, and keeps it between her paws until it activates *or she bumps it). The plastic 'holey' covering let's her get a good grip on it, and it is a little larger than a baseball. Good quality plastic/soft rubber covering.
It charges quickly, and has unpredictable jumps and rolls unlike some others that just run on 1 track. She would play with it all day if I let her. She brings it to me to play with her, but it doesn't really need a human's intervention. A tooth will occasionally hit the small control and turn it off, but no concerns about it falling apart or her ripping it apart. Highly recomment
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Reviewed in the United States on February 26, 2026
★★★★★ 3
Not as durable as it could be, but fun
Color: Yellow
Our 55 lb lab has fun with this toy, but it's not lasting long. The ball itself is hard plastic and the yellow rubber wrap around it has already been chewed through n several places. It did come with a fabric cover, but that, too, is not durable. Would not recommend leaving with your dog unattended as they could eat the rubber off in no time. But to bring out occasionally while watching the dog, which is what we do, it does provide some fun for all!
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Reviewed in the United States on May 17, 2026
★★★★★ 4
Durable enough for my dog's tough play. She loves this interactive ball.
Color: Blue, Color: Blue
I love these interactive ball toys. My dog gets so excited and engaged with them. I went through 3 of them that my dog destroyed in a short time. They simply were not durable enough for her tough play. So far, we have had this one for a few weeks, it is holding up and withstands her chewing and rough play, and she loves it. It keeps her busy for a long time.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Great toy for old dogs
Color: Yellow
Great to bring a old dog back to his old self. Have a 10 yr old who has a little shoulder pain but needs a little light excitement. This did the trick, he paws it a little and enjoys moving it around. It can take his biteing without breaking.
Hopeing our small dog whos afraid of it will watch and get over her fear. She learns everything from him.
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Reviewed in the United States on May 6, 2026