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Description
mIHC Dye (TSA770) Two-step MethodProduct Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 770 component A, TSA770 dye component B, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.2 ★★★★★
Based on 238 reviews
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Product Reviews
★★★★★ 5
Best quality
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
My dog absolutely loves these, I love that the filling isn't all dried out and crusty like the other brands. This will keep her busy for 2-3 a day and last her a few days. I do encourage these or rainy days for her. I pay 10 dollars for a lick bone at tractor supply. By that time I need a few more so honestly the price is right for the 3 pack.
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Durable and a Favorite!
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large, Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
I *was* gonna hide them for a later date because they are great for keeping them busy and quiet! But the Amazon box got delivered and my Pittie, Whiskey, was very excited about it... that girl's got a great nose! Through a cardboard box, a sealed bag, and shrink wrap, she knew the best filled bones were in there! She chose the beef one. Otto, my Heinz-57, chose the peanut butter. The bacon and cheese will act as mediator so when they steal and switch, there's always a 3rd bone to keep the peace.
These Pawstruck bones don't splinter like the Ol' Roy ones from Walmart (see photo, Ol' Roy is the darker one). Sure, they can manage to break off a few dull, tiny bone chips, but nothing that will hurt them, even if swallowed. Which, in a way is good; hard to break teeth if the bone gives just right. Whiskey is a mega power chewer and can destroy just about anything, but these hold up quite well and don't cause any gastrointestinal upset.
The dogs absolutely love them! I also never notice any smell or any significant mess. Very heavy duty and thick, great large size so they really have to work on them. I also like the filling ingredients much better than the corn meal and various fats seen in others.
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Reviewed in the United States on September 16, 2025
★★★★★ 5
4 Paws up! My dogs LOVE them
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large, Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
I have 2 large dogs who LOVE THESE.
A Great Pyrenees mix who’s a skinny 80lbs and a lab/terrier mix who’s a chunky 50lbs. They both love bones and can REALLY chew through them quickly so I’m really picky about what I buy them.
Here is what is important to me and how I rate and choose bones. These are my comments on this bone set specifically:
Do they break/splinter?
-They don’t splinter, I have seen smaller pieces that have broken off and when the bone gets down to the end (but this is normal with bones) at which point those pieces can be thrown away. I have never had an issue with them choking, being too small or hurting my dogs though and they are aggressive chewers so I could see this happening with some dogs and never happening with others. 4/5 stars for this.
Do they smell/leave stains on the carpet?
-Nope! No odor and they actually leave my dogs breath smelling better and their teeth cleaner! 5/5 stars
Does it upset my dog’s stomach? (My bigger guy can’t tolerate a LOT of things, he vomits often and also gets diarrhea if he eats the “wrong” thing so I have to be very cautious.)
-NOT at all! No upset tummies. This is always very, very important to me. Make sure you have lots of water nearby for your pups though, these will make them very thirsty. 5/5 stars
Do they hurt my dogs tongue, mouth or teeth?
-Nope, no issues with this. They’re able to gnaw into these aggressively without hurting themselves. 5/5 stars
Are they gone in 2 seconds?
-No! I’m always worried the “stuffed” bones will be left in the dust after the stuffing is all gone but my dogs will still keep chewing on these after the stuffing is gone. They still enjoy the bones and they last for quite some time, weeks at minimum. I can also stick some peanut butter or other treats back in the middle after they get the original stuffing all out of them to “reuse” them if it seems like they’re getting a little bored with them. 5/5 stars
Do my dogs ACTUALLY LIKE THEM?
-They love them. 5/5 stars
Price?
-Great and totally affordable. $20 for 3 of them.. so less than $7/bone. This is comparable to a bone from the pet store and these last a lot longer than something you would find there in the “fresh” grocery-store-like-section of the pet stores.
I’m an avid review reader, especially when it comes to items for my fur babies because I want the best for them. I truly love these bones and will keep buying them.
I will continue to buy these!
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Reviewed in the United States on August 26, 2019
★★★★★ 4
⭐️⭐️⭐️⭐️ Finally Found a Long-Lasting Bone That Actually Keeps My Dog Busy
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
I’ve tried a lot of filled bones and chew treats over the years, and most fall into two categories: gone in 5 minutes, or upset stomach later. These filled beef shank bones hit the sweet spot.
The bone itself is thick, dense, and real, not a thin, brittle shell. My medium-large dog is an aggressive chewer, and this keeps him occupied for a solid 30–45 minutes working on the filling, then even longer gnawing on the bone afterward.
The filling smells fresh and appetizing (not rancid or overly greasy), and my dog goes crazy for it. We’ve tried the cheese-style and peanut butter–style fillings, and both were a big hit. No crumbly mess, no weird residue, and no digestive issues so far.
What I really appreciate is that these feel safer than many cheaper alternatives. The edges don’t splinter, and the bone wears down gradually instead of cracking.
Why I’ll keep buying these:
• Long-lasting even for power chewers
• Real beef shank bone, not pressed junk
• Filling dogs actually care about
• Helps with boredom and anxiety
• Good value compared to pet store brands
Minor cons:
• Can get a little smelly after heavy chewing
• I supervise and take it away when it gets small (as with any bone)
If you’re tired of wasting money on chews that disappear in minutes, these are a fantastic upgrade. My dog is obsessed, and I finally feel good about what I’m giving him.
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Reviewed in the United States on February 5, 2026
★★★★★ 5
Best bones out there
Flavor Name: Variety Packs, Size: 3 Pack, Set name: Large
These are the best bones I’ve ever bought. I have a lab puppy and as you can imagine most bones don’t last long around here, but this one keeps him entertained for days.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 4, 2026
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